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The two protein bands from 40 kDa to thirty kDa have been absent right after 24 h incubation at 45 °C. For the rest of the bands, many concentrations of all 3 proteases retained protein bands corresponding to their original molar masses (Determine 6a,b). The existence of bands at very low molecular weight in fish roe in advance of hydrolysis was unpredicted. Inside the report by Ghelichi et al. [8], minor peaks of a comparatively low molecular body weight range (three–fourteen kDa) ended up observed in defatted carp roe homogenate, possibly due to an autolytic approach occurring in fish roe and/or perhaps the defatting method bringing about the formation of very low-molecular-pounds peptides.
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